The optimized mobile phase was EtOH/aqueous ammonium acetate buffer (92.5/7.5,v/v) at pH 6.8. The effect of EtOH percentage, buffer concentration, pH, column oven temperature and flow rate on chromatographic parameters was systematically investigated.
The optimized mobilephase was EtOH/aqueous ammonium acetate buffer (92.5/7.5,v/v) at pH 6.8. The effect of EtOH percentage, buffer concentration, pH, column oven temperature and flow rate on chromatographic parameterswas systematically investigated.