Sequential inoculations with T. delbrueckii AWRI1152, P. kudriavzevii AWRI1220, or Z. bailii AWRI1578 produced less acetic acid than S. cerevisiae AWRI1631 alone. Similar to T. delbrueckii AWRI1152, and despite consuming less sugar than S. cerevisiae AWRI1631 alone, C. stellata AWRI1159 produced the highest glycerol concentration in sequential fermentations.