Preparation of plant extract and fish sample
Fresh leaves of chaplu (Piper sarmentusum),
mulberry (Morus alba) and rang chuet (Thunbergia
laurifolia Lindl.) with average size (6×10 cm.) were
obtained from a farm in Pattani. The leaves from
all plants were washed, dehydrated in a dry oven
at 50 o
C for 15 h and pulverized into coarse
powder. The dried plant powder was kept in a
polyethylene bag. Each dried plant powder
(1%w/v) was extracted with hot distilled water at
95o
C for 10 min. The crude plant extract was
filtrated and cooled at 4 o
C. Tilapia fillets were
dipped with crude extract of each plant (1%w/v)
for 10 min at 4 o
C. After treatment, fillets were
drained for 10 min. Then fillets of each treatment
were placed in pouch. The control samples were
soaked in distilled water under the same
condition. All samples were taken for microbiological,
chemical and sensory analyses every 3
days for up to 15 days