2.2. Pea protein isolate
Isolate was produced by extracting pea flour in water at room temperature (ratio 1:15 w/w) and pH 7.5
followed by purification with UF/DF using 50 kDa hollow fibres membranes. The UF/DF sequence was a
UF step with a volume concentration ratio (VCR) 5 and a discontinuous DF step with a re-VCR 5. The
resulting pea protein isolate was lyophilized and placed in aluminium pouches which were hermetically
sealed and stored at 4°C until used [16].