Before the first harvest, the roots appeared to be healthy. There was no indication of nutrient deficiency during the initial harvests. However, by the fourth harvest nutrient deficiencies were evident, especially in the second hydroponic tank of each set, indicating that some nutrient or nutrients became limiting as water traveled a distance of 61 m through each set of two hydroponic tanks. The deficiency was manifested as chlorosis of the leaves. Initially there was a large reservoir of nutrients in the system as it had been operating for some time without plants, and no deficiencies appeared early in the trial when the fish were not fed during the clarifier upgrade. However, during the production of four consecutive batches of basil, nutrient depletion occurred. During this period, the ratio between daily feed input and plant growing area was 81.4 g/day/m2, which was higher than the optimum ratio of 57 g/day/m2 for Bibb lettuce. Batch production of basil exceeded the nutrient generation capacity of the system. The cropping system was therefore changed to a staggered production to moderate nutrient uptake.