16S-r-RNA gene in recombination plasmid library (collection of 16S-rRNA genes in pGEM-T vector) were amplified again using M13F and M13R primer (Moffett et al. 2000) to obtain individual 16S-rRNA genes.
16S-r-RNA gene in recombination plasmid library(collection of 16S-rRNA genes in pGEM-T vector) wereamplified again using M13F and M13R primer (Moffett et al.2000) to obtain individual 16S-rRNA genes.