-Rapid Communication
-Isolation and Characterization of a Vitellogenesis-inhibiting Factor from Sinus Glands of the Lobster,Homarus americanus
a vitellogenesis-inhibiting(VI)factor has been characterized from extracts of lobster sinus gland using an in vivo bioassay developed in the shrimp.After a two-step purification by reversed phase high-performance liquid chromatography (RP-HPLC),the bioactivity is recovered in one symmetrical peak.Sodium dodecyl sulfate-urea-polyacrylamide gel elelctrophoresis (SDS-urea-PAGE)of the bioactive fractions reveals,after silver staining,the presence of only one band corresponding to a 7-8 kD peptide