The free radical scavenging activity of all the extracts was evaluated by 1, 1-diphenyl-2-
picryl-hydrazyl (DPPH) according to the previously reported method.[18] Briefly, an 0.1mm
solution of DPPH in methanol was prepared, and 1mL of this solution was added to 3 mL of
the solution of all extracts in methanol at different concentration(125,250,500
&1000μg/mL).The mixtures were shaken vigorously and allowed to stand at room
temperature for 30 minutes. Then the absorbances were measured at 517 nm using a UV-VIS
spectrophotometer (Genesys 10UV: Thermo electron corporation).Ascorbic acid was used as
the reference. Lower absorbance values of reaction mixture indicate higher free radical
scavenging activity. The capability to scavenging the DPPH radical was calculated by using
the following formula.