เชื้อ was identified and characterized from soil entomopathogenic nematodes collected from the South Korean locations reported in our previous study (KACC accession number 91627P) (Jang et al., 2012). อาหารเลี้ยงเชื้อแบคทีเรีย was routinely maintained in Luria-Bertani (LB) broth (0.5% yeast extract, 1% NaCl, 1% tryptone) and incubated at 28 ± 2°C for 48 h to prepare a pre-culture with an
initial optical density of 0.6 at 600 nm. The strain was cultured on LB agar plant and in broth for both short- and long-term storage and subsequent use in experiments.