The best reduction of fibril size and a smooth passing of flow through homogenizer were found when the concentration was 1% for both enzymes. Nanofibers pretreated with 1% A enzyme had a diameter of 38–42 nm after three passes. A combination of bio-treatment with OS1, fungi isolated from elm tree infected with Dutch elm disease, and high-shear refining had been applied by Janardhnan and Sain (2011) on bleached kraft pulp to fibrillate NFC.