Samplesfor each timeetemperature point were exposed to isothermalheating by fully immersing them in a circulating oil bath (±0.05 C).Samples were removed after 5e35 min at 95 C; 1e10 min at100 C; 0.5e2.5 min at 105 C; and 15e60 s at 110 C. The come-uptime for the samples (<5 s) was excluded from the total heatingtime. At the end of heating, capillary tubes were cooled on iceslurries and washed with ethanol. Before microbial analysis, bothends of the capillary tubes were aseptically cut and each samplewas serially diluted in 0.9 ml 0.1% peptone water (Merck, Darmstadt,Germany). Each treatment was repeated with two sporebatches (n ¼ 6).
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