Minimum inhibition concentrations of the extracts were
evaluated for the bacterial strains which were determined as
being sensitive to the extracts in the disc diffusion assay.
A broth macrodilution method was used, as previously described
by Nakamura et al. (1999) with a slight modification.
Serial twofold dilutions of each extract were prepared in 10%
(v/v) dimethylsulfoxide (DMSO), and 30 mL of each dilution
was added to 3 mL of MuellereHinton broth. These were
inoculated with 30 mL of culture of the test bacterial strains.
After incubation of the cultures at 37 C, the MIC value wasdetermined as the lowest concentration of the extract that demonstrated
no visible growth.