Sample solution wasinjected through a 20l loop and separated on the Phenomenex
Luna-Silca column (3 m silca, 4.6mm×150mm)
at ambient temperature. Isocratic elution was performed withacetone–hexane (82:18, v/v) mobile phase at a flow rate
of 1.2 ml/min, and the detection wavelength was kept at
474 nm. Carotenoids were analyzed by UV spectrophotometer
(Pharmacia Biotech, Ultraspec 2000, Sweden) at 470 nm.
The total amount of extracted lipid was weighed after evaporating
acetone in sample solution using a dryer at 110 ◦C.