Wing morphometric traits were assessed and compared in A.
funestus mosquitoes collected in nine ecological zones belonging to
five different bioclimatic domains of Cameroon (Olivry, 1986),
encompassing most of the bio-geographic diversity of the country,
ranging from the northern arid savannas to the evergreen
rainforest in the south (Fig. 1, Table 1). To reduce possible local
effects and increase the number of specimens, mosquitoes were
collected from 2 to 7 villages in each zone (average distance
between villages within zone = 7.83 km). Adult females A. funestus
were captured by day-time spraying aerosols of pyrethroid
insecticides inside human dwellings (Service, 1993). Anopheline
mosquitoes were identified using morphological identification
keys (Gillies and de Meillon, 1968). Ovaries from half-gravid A.
funestus females were dissected and stored in Carnoy fixative
solution (3 parts of 100% ethanol: 1 part glacial acetic acid by
volume) for subsequent cytogenetic analysis. Carcasses were
stored individually in labeled tubes containing a desiccant and
kept at 20 8C.
Wing morphometric traits were assessed and compared in A.funestus mosquitoes collected in nine ecological zones belonging tofive different bioclimatic domains of Cameroon (Olivry, 1986),encompassing most of the bio-geographic diversity of the country,ranging from the northern arid savannas to the evergreenrainforest in the south (Fig. 1, Table 1). To reduce possible localeffects and increase the number of specimens, mosquitoes werecollected from 2 to 7 villages in each zone (average distancebetween villages within zone = 7.83 km). Adult females A. funestuswere captured by day-time spraying aerosols of pyrethroidinsecticides inside human dwellings (Service, 1993). Anophelinemosquitoes were identified using morphological identificationkeys (Gillies and de Meillon, 1968). Ovaries from half-gravid A.funestus females were dissected and stored in Carnoy fixativesolution (3 parts of 100% ethanol: 1 part glacial acetic acid byvolume) for subsequent cytogenetic analysis. Carcasses werestored individually in labeled tubes containing a desiccant andkept at 20 8C.
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