Table
1
–
Central
Composite
Design
(CCD)
for
two
variables
for
preparation
of
mate
bark
extracts.
Assay
Codified
Reals
TPCa
(mg
CAE/
mL)
X1 X2 Temperature
(
◦C)
Time
(min)
1 −1 −1 70 3 1.4
2 +1 −1 95 3 1.5
3 −1 +1 70 10 1.4
4 +1 +1 95 10 1.5
5 − 0 64.8 6.5 1.3
6
+
0
100.2
6.5
1.3
7
0
−
82.5
1.5
1.5
8
0
+
82.5
11.5
1.5
9 0 0 82.5 6.5 1.5
10 0
0
82.5
6.5
1.6
11
0
0
82.5
6.5
1.5
12 0 0 82.5 6.5 1.6
13 0 0 82.5 6.5 1.5
CAE, chlorogenic acid equivalent.
a Total polyphenol content.