we found LRVs of WM = 3.06 (+/−0.36) and PUR = 3.39 (+/−0.24).The surrogate virus MS2 was also tested for infectivity using thesame EPA#1 samples, yielding LRVs for WM = 3.65 (+/−0.57) andfor PUR = 3.1 (+/−0.03). By contrast, in the experiments involvingthe turbid water matrix (EPA #2, Fig. 5B), we observed the fol-lowing results. Quantification of HEV particle decay using qRT-PCRrevealed LRVs for WM = 1.53 (+/−0.46) and for PUR = 0.84 (+/−0.14).For the MS2 virus, we found LRVs for WM = 1.43 (+/−0.79) andfor PUR = 1.26 (+/−0.67). MS2 infectivity assays showed LRVs forWM = 2.36 (+/−1.75) and for PUR = 2.3 (+/−0.46).Two-way ANOVA analysis were done to evaluate difference inthe LRV between methods (PUR and WM) and between viruses(HEV and MS2) and their interaction. To satisfy the requirements ofthe ANOVA test we evaluate normality of the residues with Shapiro-Wilk test, (EPA #1 p = 0.56; EPA #2 p = 0.82); and the homogeneityof the variances with Bartlett test, (EPA #1 p = 0.69; EPA #2 p = 0.75).The results show that only in EPA #1 water matrices the treatment had a different effect between viruses (p