Enzyme assays
Endoglucanase activity was determined in
the form of carboxymethyl cellulase activity (CMCase
activity) as described by Mandel (1975). Exoglucanase
was determined in the form of filter paper activity
(FPase activity) as described by Ghose (1987). One
unit of CMCase and FPase was defined as the amount
of enzyme that released 1 µmol of glucose equivalent
per minute under assay condition.
Glucoamylase activity was determined by
dinitrosalicylic acid method of Miller (1959). One unit
of glucoamylase was defined as the amount of enzyme
necessary to produce reducing sugars equivalent to 1
µmol of reducing sugar as glucose per minute at 40 °C.