Differentiation of shoot bud from shoot tips was observed in 45% cultures on MS medium supplemented with 1.5 mg/L on concentration of BAP (Table 2). Kenetin was found most effective in differentiation of shoot buds from shoot tips in comparison to BAP (Table 2). However, Manickam et al., (2000) recorded multiple shoot formation in BAP 4.44 mm. Khattar and Mohan Ram (1982) encountered regeneration in Sesbania spp directly and indirectly on B5. However regeneration in MS medium is equally directed towards both indirect and direct organogenesis.