Collagen is the most abundant and unique protein in vertebrates, and has a wide range of applications in healthy food, cosmetics, biomaterial and pharmaceutical industries. Presently there are at least 19 variants of collagen. Type I collagen is found in all connective tissues, especially in mammals. Collagen from aquatic source has received much attention from many countries, although there are many reports available in skin and bone collagen from marine organisms. Therefore, the present result of O. nepa collagen was confirmed as type I collagen based on the results of SDS-PAGE and FT-IR analysis. Since, the mantis shrimp (Squilla) forms a major by catch resources in India and regularly utilized as feed ingredients in aquaculture industries, the new concept “wealth from the waste”, could be used as a alternative source for biomedical applications and mammalian collagen especially type I.