benzoic acid derivative from leaves of P. diospyrifolium were
evaluated by colorimetric resazurin microtiter assay plate
(REMA) as described by Palomino et al. (2002) and Martin et al.
(2003), with modifications. Briefly, 200 mL of sterile distilled
water was distributed in the outer wells of the microplate
(Falcon 3072, Becton Dickinson, Lincoln Park, NJ, USA); extracts
and pure compound were diluted in dimethyl sulfoxide (DMSO)
(Amresco), and serial twofold dilutions from 250 to 1.9 mg/mL
were made in Middlebrook 7H9 medium supplemented with
OADC. Isoniazid was used as the reference drug at concentrations
from 1.0 to 0.007 mg/mL. One hundred microliters of each
bacterial inoculum, standardized according to 1 McFarland
turbidity and diluted 1:20 in Middlebrook 7H9 medium supplemented
with OADC
benzoic acid derivative from leaves of P. diospyrifolium were
evaluated by colorimetric resazurin microtiter assay plate
(REMA) as described by Palomino et al. (2002) and Martin et al.
(2003), with modifications. Briefly, 200 mL of sterile distilled
water was distributed in the outer wells of the microplate
(Falcon 3072, Becton Dickinson, Lincoln Park, NJ, USA); extracts
and pure compound were diluted in dimethyl sulfoxide (DMSO)
(Amresco), and serial twofold dilutions from 250 to 1.9 mg/mL
were made in Middlebrook 7H9 medium supplemented with
OADC. Isoniazid was used as the reference drug at concentrations
from 1.0 to 0.007 mg/mL. One hundred microliters of each
bacterial inoculum, standardized according to 1 McFarland
turbidity and diluted 1:20 in Middlebrook 7H9 medium supplemented
with OADC
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