2.7. Bacteria staining methods
Bacteria captured on the surface of pSi were visualized with
dye staining methods. The staining reagent was prepared by
mixing 3 μl of SYTO9 and 3 μl of propidium iodide (pi) reagent
solution. The mixture was then diluted with 1 ml of sterilized ultrapure
water. The staining reagent was injected into the micro-
fluidic channel and incubated in a dark room for 15 min. After the
excess amount of dye was removed by ultrapure water, the stained
bacteria were observed with a fluorescent microscopy equipped
with a CCD camera. The fluorescent image was acquired with a
100 objective lens under blue light excitation