Nanoporous cellulose film with good dimension stability was successfully prepared by multiple freeze/ thaw cycles of glycerol aftertreated cellulose hydrogel. In addition, nanoporous structure was controllable by varying the concentration of cellulose solution, concentration of aftertreatment glycerol and a number of freeze/thaw cycles. In conclusion, 30 wt% glycerol aftertreatment and 5 freeze/thaw cycles were the optimal conditions. Hydrophobic modification of nanoporous cellulose films with coconut oil was found to decrease water wettability of nanocellulose film. As a result, modified nanoporous cellulose film exhibited affinity to cholesterol. It would therefore be possible that coconut oil modified nanoporous cellulose would offer a dietary fiber candidate with high performance of cholesterol removal.