Nowadays, phytoremediation using MA mainly focused on the use of immobilized culture. Efforts on the application of suspended MA phytoremediation became less apparent due to the difficulties in biomass separation and culture maintenance. However in this research, the focus on the extent of multi-trophic system by means of using suspended marine MA, Chlorella sp. and EM-1 in treating Lates calcarifer aquaculture wastewater was selected. Symbiotic microbial cooperation required the determination of an optimum ratio of inoculation between both bioremediators for the effective removal of pollutants. The performance of different inoculation ratio of EM and MA was evaluated. Furthermore, a detail kinetics study on nutrient removal was done in order to establish design
parameters and protocols for the multi-trophic system. Additionally, this study was aimed at integrating EM biofilter and MA photobioreactor into an innovative solution of zero discharge in
aquaculture wastewater treatment. Finally, the output of this study will help to unveil the potential of multi-trophic treatment technology especially in recirculating aquaculture wastewater treatment
system as well as enhancing the development of clean and green technology