The mixture was heated in a waterbath at
100 C for 1 h in a nitrogen atmosphere. After cooling
to room temperature, 1 ml of hexane and 1 ml of distilled
water were added to extract the fatty acid methyl ester.
The hexane phase was collected into a vial. All FAMEs
were separated and quantified using a gas chromatograph
equipped with autosampler and on-flame ionization
detector (