In conclusion, mutations in BnFUS3 outside of the B3 domain,
result in reduced levels of seed oil that are related to the transcriptional
suppression of specific genes encoding key enzymes of
pathways upstream of FA synthesis (Fig. 6). The selective downregulation
of enzymes participating in sucrose transport and
metabolism, as well as glycolysis, is an indication of a diminished
carbon pool available for the synthesis of FA and ultimately oil
production. Based on these observations, it is therefore suggested
that targeted manipulations of FUS3 can be used as a tool to influence
oil accumulation in the economically important species
B. napus.