This study was supported by the project ‘‘WW-SIP - From Urban Wastewater Treatment Plant to Self-Sustainable Integrated Platform for Wastewater Refinement’’ (LIFE10 ENV/IT/000308). Ana Paula Batista acknowledges the Post-Doc Grant SFRH/BPD/84812/2012 from FCT – Portugal. Lucas Ambrosano thanks Capes Foundation, Ministry of Education – Brazil for his research grant (99999.012781/2013-04). The authors thank Graça Gomes and Natércia Santos for microalgae culture maintenance and laboratory assistance.