The fluorescence spectroscopy (FS) is increasingly used in petroleum technology due the availability of better optical detection techniques, because FS offers high sensitivity, good diagnostic potential, and relatively simple instrumentation. Absorption and fluorescence spectrum of crude oil was studied at room temperature using optical path cuvette 1 cm. In this work, the absorption spectra were scanned (190-1100) nm while the emission spectra were obtained at different excitation wavelengths (266, 337, 420, and 488) nm. Crude oil was diluted by absolute ethanol to prepare different concentrations of crude oil. Ethanol as a solvent was used to dilute crude oil to obtain a solution transparent and the light could be transmitted. This work considers as an additional spectroscopic tool to develop into a fast responsive system for charchtrize the crude oil.