The hydrolysate was adjusted to initial pH 6 with 6 M
KOH and then separated (10,000 rpm, 30 min) using centrifuge
separator (Westfalia Separator Mineral oil Systems GmbH D-
59302, Germany) to remove any precipitate formed before using
it as substrate. The dry LB ingredients were added to the hydrolysate
and were not sterilized for fermentation