One of the coauthors (Uraiwan Chatchawan) with expertise in epidemiology and biostatistics analyzed the data and was completely were performed on the basis of the intention-to-treat principle. All outcome measures are presented as means ± standard deviations (SD). The independent samples t-test was used for comparisons between the groups, and the dependent samples t-test was used for comparisons between before and after the intervention. Interaction effect were assessed by the ordinary least squares (OLS) method13). We used a cross analysis (Student’s t-test) to compare the mean change in score (post-pre) because the primary outcomes(VO2 max) was normally distributed (Shapiro-Wilk W test; p>0.05)14, 15). In case of significant period × treatment interaction, the second-period data were not used for the analysis16).