The precipitation of lignin was conducted overnight using (72% v/v) H2SO4 at pH 2.0.At higher pH values, precipitation was not detected, as reported by Minu et al. (2012). Whatman No. 4 filter paper was employed for vacuum filtration. The filtered liquid was analyzed for cellulose, hemicellulose and acid soluble lignin, as described in the Section 3.3.