500 g of raw rubber (cup lumps) were cut into small pieces (crumbs) in order to increase the surface area and soaked for 24 hours in the antimicrobial agent prepared. The rubber crumbs were rinsed and dried in an oven at 50 0C for about 3 to 4 days until the crumb reach constant weight. Then, treated rubber was used to develop vulcanized rubber according to formula in Table 1. The vulcanized rubber sheets were cut into dumbbell shapes in order to determine the tensile strength, percentage of the resilience, and the rubber hardness according to the International Rubber Hardness Degree (IRHD). The tests were carried out by using Automated Materials Testing System 6.05 Model 1011 (Instron Corp., Series IX, USA) at a crosshead speed of 500 mm/min (Hoven et. al., 2004).