More specifically, the influence of enhanced H+ that is derived from
benzoic acid may directly alter the renal compensatory
mechanism driven towards maintenance of plasma pH.
Given that bone weight and Ca concentration in bone ash
were affected by benzoic acid, a change in bone resorption
induced to buffer the increase in acid absorption is likely. If
one considers that the normal resorption of AA from the
glomerular filtrate is associated with cotransport of Na, a
conjugation of free glycine with benzoic acid may have
indirectly increased Na excretion in urine of the pigs fed the
benzoic acid-containing diets. Furthermore, the increased
urinary Cl excretion is likely a result of several other physiological
adaptations including the increased urinary Na loss,
the increased H+ concentration in the distal tubules, and the
increased bicarbonate resorption; however, the latter two
were not measured in the present study. Therefore, the
reduction in Chlorine concentration in the femur of pigs fed the
benzoic acid-containing diets in this study is likely a direct
reflection of the increased urinary excretion of Chlorine which in
itself reflects the new