Please store the antibodies in the original tube at -20°C and do not aliquot as this may introduce variables and waste antibody. The antibodies are provided in a buffer that contains 50% glycerol and do not freeze under the recommended storage conditions. This prevents degradation of the antibody due to repeated freeze-thaw cycles. Conjugated antibodies may indicate storage at 4°C, please store as suggested on the product datasheet. - See more at: http://www.cellsignal.com/about/faq.jsp#sthash.kIpwBMO3.dpuf