Much response was not obtained from leaf explants. The MS medium supplemented with NAA (1 mg/mL) showed friable callus in 62% of the leaf explants. NAA (0.5 mg) induced 45% callus formation. Callus induction was observed after 6 days of culture. MS medium without activated charcoal produced green callus initially, which turned brown in 3-5 days. The chemicals produced by the brown callus changed the medium to yellow color. When activated charcoal (1g/L) was added to MS medium, green callus was obtained. Friable callus was also obtained when the culture bottles were stored in complete darkness. Hormones other than NAA did not show any significant induction of callus compared to control.