Chromatographic conditions for the elution of aspartame as
optimized by Arora, Shendurse, Sharma, Wadhwa, and Singh
(2013) was used. However, chromatographic conditions for the
elution of neotame were optimized as following:
Three different mobile phases were chosen for the elution of
neotame viz. 0.09% TFA:water:acetonitrile (70:30, 80:20, 60:40).
0.09% TFA:water:acetonitrile (60:40) gave the best resolution as
compared to the other two mobile phases. Three flow rates (0.6,
0.8 and 1.0 ml/min) of the mobile phase were assayed for the estimation
of neotame. The neotame peak merged with the product
matrix peak at a flow rate of 0.8 and 1.0 ml/min, hence, a flow rate
0.6 ml/min was selected for the analysis. At this flow rate the
retention time increased and better resolution was obtained as
compared to the flow rate of 0.8 and 1.0 ml/min.
In order to select the wavelength for analysis of neotame, a reference
standard solution of neotame was injected and UV spectra
were scanned in the UV range (200–380 nm). Neotame showed
kmax at 210 nm.
Chromatographic conditions for the elution of aspartame asoptimized by Arora, Shendurse, Sharma, Wadhwa, and Singh(2013) was used. However, chromatographic conditions for theelution of neotame were optimized as following:Three different mobile phases were chosen for the elution ofneotame viz. 0.09% TFA:water:acetonitrile (70:30, 80:20, 60:40).0.09% TFA:water:acetonitrile (60:40) gave the best resolution ascompared to the other two mobile phases. Three flow rates (0.6,0.8 and 1.0 ml/min) of the mobile phase were assayed for the estimationof neotame. The neotame peak merged with the productmatrix peak at a flow rate of 0.8 and 1.0 ml/min, hence, a flow rate0.6 ml/min was selected for the analysis. At this flow rate theretention time increased and better resolution was obtained ascompared to the flow rate of 0.8 and 1.0 ml/min.In order to select the wavelength for analysis of neotame, a referencestandard solution of neotame was injected and UV spectrawere scanned in the UV range (200–380 nm). Neotame showedkmax at 210 nm.
การแปล กรุณารอสักครู่..