2.4. Phytotoxicity
Potential phytotoxicity was quantified by two germination bioassays. The first bioassay, conducted following the Italian official method UNI 10780:1998, was performed using the commercial seeds of cress cv. Alenois (Lepidium sativum L.). The compost (200 g) was moistened at 85% with deionized water and left standing for 2 h. It was further centrifuged (6000 rpm, 15 min) and filtered through a filter paper with the help of a vacuum-pump. A control treatment was prepared with deionized water. The compost extract obtained was used at different dilution rates. For each experimental unit (bin) three samples were analyzed. Ten seeds of cress were placed in 9 cm Petri plates with 5 ml of compost extract and placed in a germination chamber at 27 °C for 24 h in absence of light. In addition, a second bioassay was performed with lettuce cv. Solana (Lactuca sativa L.) seeds. In this case, the plates were incubated for 48 h at 16 °C. Differences with control in terms of number of geminated seeds and roots length were expressed in germination index (IGe; Zucconi et al., 1981). The phytotoxicity was indicated by values of IGe < 60.
2.4. PhytotoxicityPotential phytotoxicity was quantified by two germination bioassays. The first bioassay, conducted following the Italian official method UNI 10780:1998, was performed using the commercial seeds of cress cv. Alenois (Lepidium sativum L.). The compost (200 g) was moistened at 85% with deionized water and left standing for 2 h. It was further centrifuged (6000 rpm, 15 min) and filtered through a filter paper with the help of a vacuum-pump. A control treatment was prepared with deionized water. The compost extract obtained was used at different dilution rates. For each experimental unit (bin) three samples were analyzed. Ten seeds of cress were placed in 9 cm Petri plates with 5 ml of compost extract and placed in a germination chamber at 27 °C for 24 h in absence of light. In addition, a second bioassay was performed with lettuce cv. Solana (Lactuca sativa L.) seeds. In this case, the plates were incubated for 48 h at 16 °C. Differences with control in terms of number of geminated seeds and roots length were expressed in germination index (IGe; Zucconi et al., 1981). The phytotoxicity was indicated by values of IGe < 60.
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