Differen t extraction condit ions were app lied to investigate the ef fect of temperature, extraction time and
substrate–extrac tant ratio on pectin extraction from cocoa husks. Pectin was extracted from cocoa husks
using water, citric acid at pH 2.5 or 4.0, or hydrochloric acid at pH 2.5 or 4.0. Temperature, extraction time
and substrate–extractant ratio affected the yields, uronic acid contents, degrees of methylation (DM) and
degrees of acetylati on (DA) of the extracted pectins using the five extractants differently. The yields and
uronic acid contents of the extracted pectins ranged from 3.38–7.62% to 31.19–65.20%, respectively. The
DM and DA of the extracted pectins ran ged from 7.17–57.86% to 1.01–3.48%, respectively. The highest
yield of pectin (7.62%) was obtained using citric acid at pH 2.5 [1:2 5 (w/v)] at 95 C for 3.0 h. The highest
uronic acid content (65.20%) in the pectin was obtained using water [1:25 (w/v)] at 95 C for 3.0 h