The results showed Extract supernatant (CFS) of lactic acid bacteria and bifidobacteria. Can inhibit Staphylococcus aureus were approximately 0.5% to 34.2%. It also found that Supernatant extracts of lactic acid bacteria strains. Lactobacillus bulgaricus FTDC 8611 with lactic acid, acetic acid and hydrogen peroxide, and diacetyl. That can inhibit the biofilm formation of bacteria. Staphylococcus aureus as well.