Simple smear: a drop of the sediment was applied on the center of a clean grease-free slide. A clean cover slip was placed gently to avoid air bubbles and over flooding. The preparation was examined under a light microscope using •10 and •40 objectives.12
(2) Iodine smear: a drop of the sediment was mixed with a drop of Lugol’s Iodine solution and examined as in sim- ple smear