sterilization. A desired synthetic dye mixture is prepared by using
tap water and then put into an autoclave at 121 ◦C for 15 min for
sterilization to ensure that there are not any microorganisms in
the synthetic dye solution. The speed of the pump (C6-MIDI multichannel
pump, Watson Marlow SCI) is changed to provide a flow
rate between 0.351 mL/min and 0.981 mL/min in order to maintain
the required residence time. To determine the dye removal efficiency
of the microorganisms, samples are taken every two days
from each reactor and also from the orginal dye mixture and then
analyzed.