Previous studies with B. bassiana cultures showed that
blastospore production was inversely correlated to the water
activity of the liquid media with blastospore yields remaining
very low and thus making the process not suitable for largescale
production (Humphreys et al. 1989; Inch and Trinci
1987; Ypsilos and Magan 2004). Recent studies have shown
that high concentrations (1×109 blastospores mL−1) of stable,
effective blastospores of B. bassiana can be rapidly produced in
a short 2–3-day liquid culture fermentation (Mascarin et al.
2015).Within this context, the aim of this study was to improve
the yield and fitness of blastospores of two isolates of
B. bassiana, ESALQ 1432 and GHA, by evaluating the impact
of dissolved oxygen coupled with high glucose concentrations
on the growth rate, yield, stability, and insecticidal efficacy of
blastospores produced using liquid culture fermentation.
Previous studies with B. bassiana cultures showed thatblastospore production was inversely correlated to the wateractivity of the liquid media with blastospore yields remainingvery low and thus making the process not suitable for largescaleproduction (Humphreys et al. 1989; Inch and Trinci1987; Ypsilos and Magan 2004). Recent studies have shownthat high concentrations (1×109 blastospores mL−1) of stable,effective blastospores of B. bassiana can be rapidly produced ina short 2–3-day liquid culture fermentation (Mascarin et al.2015).Within this context, the aim of this study was to improvethe yield and fitness of blastospores of two isolates ofB. bassiana, ESALQ 1432 and GHA, by evaluating the impactof dissolved oxygen coupled with high glucose concentrationson the growth rate, yield, stability, and insecticidal efficacy ofblastospores produced using liquid culture fermentation.
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