centrifugation (except B1 and B2) was extracted using the PSP 1
Spin Stool kit and following the manufacturer’s instructions. For
molecular identification of FLA, amoebic plaque culture from all
samples except S2 and S12 which were consider negative because
of insufficient growth, was collected with 1ml of saline buffer, and
centrifuged for 10 min at 6000 rpm. The pellet was resuspended in
100ml of fresh saline buffer and used for DNA isolation which was
performed using Ron’s Tissue DNA Mini Kit according to the
manufacturer’s specifications.