In the 160 dah-stage group, all individuals in the initial control
group had mature ovaries with many oocytes at the vitellogenic
stage. Sixty days after treatment, gonads of the control group fish had the ovaries with mature stage oocytes (Fig. S3A, S3C), whereas
the gonads of all fish in the 37C group had small ovaries with
degenerating oocytes (Fig. S3B, S3D). After transferring to normal
temperature for three months, all fish in the control group had
well-developed ovaries with mature stage oocytes, whereas all fish
in the 37C group had small and abnormal ovaries with degenerated oocytes. The anterior one-third of the ovary was completely
devoid of germ cells and the posterior portion contained a few
degenerating yolky oocytes. This indicates that the disruption of
reproduction had already started and longer time of extended