1) Semen Volume
The semen collected using AV was measured by recording the value on graduated semen collecting tube before
being transferred into the 15 mL graduated Falcon tube. The measurement of semen collected by means of
EE was also measured by reading the value on 15 mL graduated Falcon tubes (Minitube®, South Africa). All the
semen samples were labelled and then placed in a thermoflask at 37˚C immediately after collection