•Most probable number (MPN) *dilution samples were prepared *three or five tubes method *statistical in nature •Dye reduction *methyleneblue and resazurin *the time of dye reduction •Direct microscopic count *smear on to the slide, staining and counting
•Most probable number (MPN)*dilution samples were prepared*three or five tubes method*statistical in nature•Dye reduction*methyleneblue and resazurin*the time of dye reduction•Direct microscopic count*smear on to the slide, staining and counting