Polyphosphazenes are hybrid inorganic-organic polymers with tunable physicochemical and biocompatible properties, which give them a promising role in biomedical industry as biosensors, controlled release, drug delivery, scaffolding materials, and enzyme immobilization. A core/sheath nanofiber membrane was prepared by using poly phosphazene (PMPPh) via a coaxial electrospinning process to investigate the system as a support material for enzyme immobilization because of its high biocompatibility, high surface/volume ratio, and large porosity. Lipase was immobilized on the nanofiber membrane by ad• sorption. With the adsorption capacity of 20.4 mg/g and activity retention of63.7 % of the immobilized lipase, the nanofiber membrane system with polyphosphazenes showed great results to use for biomedical applications