Chromosome checks
A complete metaphase plate chromosome was counted and photographed under compound microscope (Olympus, CH30, Japan). Length of short (Ls) and long arm (Ll) on chromosome were measured by using the Adobe Photoshop software. Those values were used to calculate the length of total arm chromosome (LT) (LT=Ls+Ll). The relative length (RL) and centrometric index (CI) were estimated by the method according Chaiyasut (1989). CI = (q/p+q) as 0.50-0.59, 0.60-0.69, 0.70-0.89 and 0.90-0.99 are described as metacentric, submetacentric, acrocentric and telocentric chromosomes, respectively. All values were used for karyotype and idiogram.