3.7.3. Changes in lipid oxidation of emulsion during storage
Lipid oxidation of menhaden oil-in-water emulsion stabilised
with gelatin modified with and without oxidised tannic acid was
monitored and expressed as TBARS values during the 12 days of
storage (Fig. 4B). Generally, no TBARS were found in the oil-inwater
emulsion within the first 2 days of storage. Thereafter, the
marked increase in TBARS was observed, especially in emulsion
stabilised by control gelatin (p < 0.05). Emulsion stabilised by gelatin
modified with oxidised tannic acid had the lower TBARS
(p < 0.05). This result was in accordance with the antioxidative
activity of gelatin modified with oxidised tannic acid (Fig. 2). Antioxidative
activity of gelatin modified with or without oxidised tannic
acid increased with increasing concentration used.
Additionally, the preventive effect of gelatin at the higher level
used on lipid oxidation might be associated with the thicker film
around the oil droplet. This could prevent the penetration of oxygen
into an oil droplet more effectively. Therefore, gelatin modified
with oxidised tannic acid had the ability to retard TBARS formation
in the oil-in-water emulsion system. This result was in agreement
with Almajano and Gordon (2004) who reported that interaction of
BSA and epigallocatechin gallate (EGCG) could induce the formation
of a BSA-antioxidant adduct and cause a synergistic increase
in the oxidative stability of sunflower oil-in-water emulsion effectively,
when compared with BSA or EGCG alone.
3.7.3. Changes in lipid oxidation of emulsion during storageLipid oxidation of menhaden oil-in-water emulsion stabilisedwith gelatin modified with and without oxidised tannic acid wasmonitored and expressed as TBARS values during the 12 days ofstorage (Fig. 4B). Generally, no TBARS were found in the oil-inwateremulsion within the first 2 days of storage. Thereafter, themarked increase in TBARS was observed, especially in emulsionstabilised by control gelatin (p < 0.05). Emulsion stabilised by gelatinmodified with oxidised tannic acid had the lower TBARS(p < 0.05). This result was in accordance with the antioxidativeactivity of gelatin modified with oxidised tannic acid (Fig. 2). Antioxidativeactivity of gelatin modified with or without oxidised tannicacid increased with increasing concentration used.Additionally, the preventive effect of gelatin at the higher levelused on lipid oxidation might be associated with the thicker filmaround the oil droplet. This could prevent the penetration of oxygeninto an oil droplet more effectively. Therefore, gelatin modifiedwith oxidised tannic acid had the ability to retard TBARS formationin the oil-in-water emulsion system. This result was in agreementwith Almajano and Gordon (2004) who reported that interaction ofBSA and epigallocatechin gallate (EGCG) could induce the formationof a BSA-antioxidant adduct and cause a synergistic increasein the oxidative stability of sunflower oil-in-water emulsion effectively,when compared with BSA or EGCG alone.
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