Gel/PCR Buffer contains guanidine thiocyanate. During the procedure, always wear
a lab coat, disposable gloves, and protective goggles.
Gel/PCR Buffer (pH≤7.5, yellow color, premixed with pH indicator) reaction of gel slice or PCR product
DNA binding to membrane while contaminants remain suspended
Wash (removal of contaminants while DNA remains bound to membrane)
Elution of pure DNA which is ready for subsequent reactions