Plants of the Zn, Cd and Pb hyperaccumulator Thlaspi praecox Wulfen (Brassicaceae) inoculated or not with indigenous
arbuscular mycorrhizal (AM) fungal mixture were grown in a highly Cd, Zn and Pb contaminated substrate in order to evaluate the
functionality of symbiosis and assess the possible impact of AM colonisation on heavy metal uptake and tolerance. The results
suggest AM development in the metal hyperaccumulating T. praecox is favoured at elevated nutrient demands, e.g. during the
reproductive period. AM colonisation parameters positively correlated with total soil Cd and Pb. Colonised plants showed
significantly improved nutrient and a decreased Cd and Zn uptake as revealed by TRXRF, thus confirming the functionality of the
symbiosis. Reduced heavy metal uptake, especially at higher soil metal contents, indicates a changed metal tolerance strategy in
colonised T. praecox plants. This is to our knowledge the first report on AM colonisation of the Zn, Cd and Pb hyperaccumulator
T. praecox in a greenhouse experiment.
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